LightCycler® 2.0

​Use ​LightCycler® 2.0 when you need to feel the strength of multiplicity. Product specifications:
 
  • This instrument provides the majority of innovative and exciting features such as a completely new software or additional channels for detection in order to enhance the characteristics.
  • Optimized hardware components such as:
    • the renewed fan allows ultra-fast and accurate regulation and distribution of uniform temperature across the board for small and large capillaries.
    • improved photometric unit which measures in 32 capillaries of 6 wavelengths for a period of 5 seconds, ensuring maximum sensitivity and homogeneity of the signal.
  • Uses glass capillaries of 20 and 100 μL as well as barcode on the sample carrousel, which simplifies and accelerates the entire process.
  • Work with 6 channels for detection of 530, 560, 610, 640, 670 and 705 nm wavelength.
  • Use of 20 μL glass capillaries for accelerating cycles.
  • Used for applications such as:
    • reproducible and confidential quantification of DNA, cDNA or RNA through the technology of absolute or relative quantification.
    • easy identification and  characterization of PCR products by analyzing the melting curve and this in two ways:
      • genotyping Analysis and
      • Tm Calling Analysis
    • qualitative detection of PCRØ products.
    • multiplex tests using  HybProbe detection format.
  • Material quantification through absorption measurements.
  • Opportunity for In – Vitro diagnostic applications in combination with the In – Vitro Diagnostic kits from Roche.
  • Formats of detection:
    • SYBRØ Green I
    • HybProbe for double and multiplex tests
    • One color SimpleProbe
    • Two colors Hidrolysis Probes
    • Other formats based on work by FRET (Fluorescence Resonance Energy Transfer)
  • Possibility to compare and insight in the data during the process and between two activities.
  • Using this technology allows you to get results in less than 1 hour; it minimizes and decreases almost to zero the appearance of unspecific products during the reaction which can lead to an increased number of copies.
  • Provides reliable and wide range of concentrations as well as high sensitivity with possibility to detect a single copy in 3 pg of human genetic DNA.
For more detailed information about the product specifications and the test menu, click here.