Use LightCycler® 2.0 when you need to feel the strength of multiplicity. Product specifications:
- This instrument provides the majority of innovative and exciting features such as a completely new software or additional channels for detection in order to enhance the characteristics.
- Optimized hardware components such as:
- the renewed fan allows ultra-fast and accurate regulation and distribution of uniform temperature across the board for small and large capillaries.
- improved photometric unit which measures in 32 capillaries of 6 wavelengths for a period of 5 seconds, ensuring maximum sensitivity and homogeneity of the signal.
- Uses glass capillaries of 20 and 100 μL as well as barcode on the sample carrousel, which simplifies and accelerates the entire process.
- Work with 6 channels for detection of 530, 560, 610, 640, 670 and 705 nm wavelength.
- Use of 20 μL glass capillaries for accelerating cycles.
- Used for applications such as:
- reproducible and confidential quantification of DNA, cDNA or RNA through the technology of absolute or relative quantification.
- easy identification and characterization of PCR products by analyzing the melting curve and this in two ways:
- genotyping Analysis and
- Tm Calling Analysis
- qualitative detection of PCRØ products.
- multiplex tests using HybProbe detection format.
- Material quantification through absorption measurements.
- Opportunity for In – Vitro diagnostic applications in combination with the In – Vitro Diagnostic kits from Roche.
- Formats of detection:
- SYBRØ Green I
- HybProbe for double and multiplex tests
- One color SimpleProbe
- Two colors Hidrolysis Probes
- Other formats based on work by FRET (Fluorescence Resonance Energy Transfer)
- Possibility to compare and insight in the data during the process and between two activities.
- Using this technology allows you to get results in less than 1 hour; it minimizes and decreases almost to zero the appearance of unspecific products during the reaction which can lead to an increased number of copies.
- Provides reliable and wide range of concentrations as well as high sensitivity with possibility to detect a single copy in 3 pg of human genetic DNA.